The authors did a good job to explain background and need to use metagenomic data for microbial source tracking. Overall the manuscript is well written. All the figures and tables are easy to read. Citation of SourceTracker should be added in line 75 after its first introduction, instead of line 80.I would suggest that the authors also discuss 1) if there are other softwares that can be used for microbial source tracking using metagenomic data and 2) if so, what are the pros and cons of existing softwares and the unique features of mSourceTracker.There are some formatting and language issues that should be addressed.Line 60, "_ _" before "ecosystem function" should be deleted.Line 77, a comma should be added before "which". Actually, please check the usage of "which" throughout the whole manuscript.Line 93, change "allows" to "allow"Line 94, change "eukaryotes" to "eukaryota"Line 115, change the comma after "this study" to something like "this study, which includes"Line 150, change "which" to "that" after "Samples"
Data acquisition on this project will likely be carried out at night to minimize surface noise associate with wind and cultural activities (vehicle traffic). It is imperative that our seismic activities minimally impact current and future agricultural activities within the affected 1 square mile. With that, a digital tracking log is being built to help guide the vibratory source (13,000 lb center articulated, four-wheel-drive vehicle with >3 psi ground pressure) around the site, avoiding fences, ditches, pipelines, etc. and minimizing ground compaction and number of miles traveled through tilled farm fields. It is our assumption that limited-sight conditions will likely be present during some of the 12 different 3-D surveys throughout this six-year program. The vibrator will be outfitted with a notebook computer and high-resolution GPS, which will direct the operator from point to point when weather or night operations permit little or no visibility. To build this digital terrain map David Laflen and Brett Bennett, KGS researchers, developed a DGPS mapping system which included a six-wheel-drive ATV, Trimble DGPS, Garmin GPS, and notebook computer (Figure 1). Aided by orthophotos and topo maps the two researchers drove and digitally mapped the "best" vibrator route (Figure 2). Key priorities were minimal ground disturbance and best path around obstacles in all weather conditions. The digital mapping system was developed and assembled at the Kansas Geological Survey's fabrication facility. This modular system uses custom software interfaced to two GPS receivers, allowing real time placement and guidance referenced to orthophotos (digital, high-resolution aerophotos) and digital topo maps.Figure 1--DGPS Mapping System. A larger version of this photo is available.Figure 2--Route Mapping. A larger version of this photo is available. A preliminary tracking log provides an overview of the route most easily traversed by the ATV and that the researchers felt would accommodate the vibrator in all weather (Figure 3). The red lines track the path followed by the digital mapping unit. The blue dots are the ideal shot station locations. With the western two-thirds completed, all but about 10% of the shot stations are easily accessible. It is anticipated that with some minor dozer work this missing 10% in the western two-thirds could be reduced to less than 5%. Once this preliminary mapping is complete, manual editing will produce a uniform route with location accuracy around 5 cm.Figure 3--Preliminary Tracking Log. A larger version of this photo is available.
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